Use the Following to Answer Questions

Multiple Choice

Use the following to answer questions
A scientist carries out a ligation reaction designed to insert a foreign piece of DNA into a plasmid that contains the front end of the lacZ gene within the multiple cloning site (MCS) . He carries out three transformations in parallel with lacZ^- bacteria. The three transformations (i, ii, and iii) contain the following:
i. sterile water
ii. a sample of the original plasmid
iii. the ligation reaction
He then plates the transformations on medium containing X-gal and ampicillin.
-What results would indicate the scientist should proceed to culture colonies from (iii) that might contain the desired clone?

A) Many blue colonies on all three plates.
B) White colonies on plates (i) and (ii) and predominantly blue colonies on plate (iii) .
C) No colonies on plate (i) , white colonies on plate (ii) , and predominantly blue colonies on plate (iii) .
D) No colonies on plate (i) , blue colonies on plate (ii) , and predominantly white colonies on plate (iii) .
E) No colonies on plate (i) , blue colonies on plate (ii) , and predominantly blue colonies on plate (iii) .