If the Optimal PH for Taq Polymerase Is About 7

Multiple Choice

If the optimal pH for Taq polymerase is about 7.2, why are the reaction buffers manufactured to have a pH of about 8.5?

A) Unincorporated nucleotides are unstable at neutral to acidic pHs.
B) Hydrogen bonds form poorly at neutral to acidic pHs.
C) Optimal reaction conditions for Taq polymerase are generally not used because rapid incorporation of nucleotides into a growing DNA chains leads to more errors.
D) pH is temperature dependent, and at the optimal temperature for Taq polymerase to function, the buffer pH will decrease.