Review the PCR Protocol Herein and Identify Its Name

Multiple Choice

Review the PCR protocol herein and identify its name. A single- stranded probe with internal complementary sequences folds on itself into a "hairpin" shape bringing a fluorescent molecule at one end of the strand in proximity to the quencher molecule at the other end of the strand. After PCR product is formed, during the next denaturation step the probe becomes single- stranded, binds to PCR target DNA, and separates the fluorescent molecule from the quencher molecule. PCR product is detected as increased fluorescence.

A) Molecular beacon PCR
B) 5' RACE PCR
C) Reverse transcriptase PCR
D) Branched chain PCR
E) TaqMan PCR