Question 1

In general, DNA replicates semiconservatively and bidirectionally.

Accepted Answer

A) True 
 B)False

Question 2

DNA replicates conservatively, which means that one of the two daughter double helices is "old" and the other is "new."

Accepted Answer

A) True 
 B)False

Question 3

Below is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the
locations specified by the numbers.
  1() Which end (5' or 3') of the molecule is here?
2() Which enzyme is probably functioning here to deal with supercoils in the DNA?
3() Which enzyme is probably functioning here to unwind the DNA?
4() Which nucleic acid is probably depicted here?
5() What are these short DNA fragments usually called?
6() Which enzyme probably functions here to couple these two newly synthesized fragments of DNA?
7() Is this strand the leading or lagging strand?
8() Which end (5' or 3') of the molecule is here?

Accepted Answer

The answer of Below is a diagram of DNA replication...

Question 4

Assume that you are microscopically examining mitotic metaphase cells of an
organism with a 2n chromosome number of 4 (one pair metacentric and one pair
telocentric). Assume also that the cell passed through one S phase labeling (innermost phosphate of dTTP radioactive) just prior to the period of observation. Assuming that the circle below represents a cell, draw its chromosomes and the autoradiographic
pattern you would expect to see.

Accepted Answer

The answer of Assume that you are microscopically examining mitotic...

Question 5

What is the name of the replication unit in prokaryotes, and how does it differ in eukaryotes?

Accepted Answer

The answer of What is the name of the replication...

Question 6

As unwinding of the helix occurs during DNA replication, tension is created ahead of
the replication fork. Describe the nature of this tension and state the manner in which it is resolved.

Accepted Answer

The answer of As unwinding of the helix occurs during...

Question 7

During DNA replication, what is the function of RNA primase?

Accepted Answer

The answer of During DNA replication, what is the function...

Question 8

Briefly describe what is meant by the term autoradiography and identify a classic
experiment that used autoradiography to determine the replicative nature of DNA in eukaryotes.

Accepted Answer

The answer of Briefly describe what is meant by the...

Question 9

Which cluster of terms accurately reflects the nature of DNA replication in prokaryotes?

Accepted Answer

A)  fixed point of initiation, bidirectional, conservative 
B)  fixed point of initiation, unidirectional, conservative 
C)  fixed point of initiation, bidirectional, semiconservative 
D)  random point of initiation, unidirectional, semiconservative 
E)  random point of initiation, bidirectional, semiconservative

Question 10

Structures located at the ends of eukaryotic chromosomes are called
A) recessive inversions
B) centromeres
C) telomerases
D) telomeres
E) permissive mutations

Accepted Answer

The answer of Structures located at the ends of eukaryotic...

Question 11

The discontinuous aspect of replication of DNA in vivo is caused by

Accepted Answer

A)  trinucleotide repeats 
B)  the 5' to 3' polarity restriction 
C)  topoisomerases cutting the DNA in a random fashion 
D)  sister -chromatid exchanges 
E)  polymerase slippage

Question 12

Chromatin assembly factors (CAFs) move along with the replication fork and assemble new nucleosomes.

Accepted Answer

A) True 
 B)False

Question 13

Which terms accurately reflect the nature of replication of the chromosome in E. coli?

Accepted Answer

A)  multirepliconic and telomeric 
B)  bidirectional and multirepliconic 
C)  unidirectional and reciprocal 
D)  unidirectional and fixed point of initiation 
E)  bidirectional and fixed point of initiation

Question 14

Compare the rate of DNA replication in prokaryotes and eukaryotes.

Accepted Answer

The answer of Compare the rate of DNA replication in...

Question 15

Bacteria are dependent on telomerase to complete synthesis of their chromosome ends.

Accepted Answer

A) True 
 B)False

Question 16

DNA polymerase I is thought to add nucleotides _.

Accepted Answer

A)  on single -stranded templates without need for an RNA primer 
B)  to the 3' end of the primer 
C)  in a 5' to 5' direction 
D)  to the 5' end of the primer 
E)  in the place of the primer RNA after it is removed

Question 17

The complex of proteins that is involved in the replication of DNA is called a(n)
________.

Accepted Answer

The answer of The complex of proteins that is involved...

Question 18

What three possible models were suggested to originally describe the nature of DNA replication?

Accepted Answer

The answer of What three possible models were suggested to...

Question 19

The Meselson and Stahl experiment provided conclusive evidence for the
semiconservative replication of DNA in E. coli. What pattern of bands would occur in a CsCl gradient for conservative replication?

Accepted Answer

The answer of The Meselson and Stahl experiment provided conclusive...

Question 20

Which protein is responsible for the initial step in unwinding the DNA helix during replication of the bacterial chromosome?

Accepted Answer

The answer of Which protein is responsible for the initial...

Exam 10: DNA Replication and Recombination

In general, DNA replicates semiconservatively and bidirectionally.

DNA replicates conservatively, which means that one of the two daughter double helices is "old" and the other is "new."

What is the name of the replication unit in prokaryotes, and how does it differ in eukaryotes?

As unwinding of the helix occurs during DNA replication, tension is created ahead of the replication fork. Describe the nature of this tension and state the manner in which it is resolved.

During DNA replication, what is the function of RNA primase?

Briefly describe what is meant by the term autoradiography and identify a classic experiment that used autoradiography to determine the replicative nature of DNA in eukaryotes.

Which cluster of terms accurately reflects the nature of DNA replication in prokaryotes?

Structures located at the ends of eukaryotic chromosomes are called A) recessive inversions B) centromeres C) telomerases D) telomeres E) permissive mutations

The discontinuous aspect of replication of DNA in vivo is caused by

Chromatin assembly factors (CAFs) move along with the replication fork and assemble new nucleosomes.

Which terms accurately reflect the nature of replication of the chromosome in E. coli?

Compare the rate of DNA replication in prokaryotes and eukaryotes.

Bacteria are dependent on telomerase to complete synthesis of their chromosome ends.

DNA polymerase I is thought to add nucleotides _.

The complex of proteins that is involved in the replication of DNA is called a(n) ________.

What three possible models were suggested to originally describe the nature of DNA replication?

The Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication of DNA in E. coli. What pattern of bands would occur in a CsCl gradient for conservative replication?

Which protein is responsible for the initial step in unwinding the DNA helix during replication of the bacterial chromosome?