Which Lists the Potential Steps for Genetic Therapy for Mice

Multiple Choice

Which lists the potential steps for genetic therapy for mice with sickle cell disease (fiS/fiS) in the correct order? I. Differentiation of iPS cells into hematopoietic progenitor (HP) cells by infecting with retrovirus expressing HoxB4.
II. Reprogramming tail fibroblasts into induced pluripotent stem (iPS) cells by infecting them with vectors containing transcription factors.
III. Infection with a viral vector expressing Cre recombinase to remove transcription factor(s) from f iPS cells.
IV. Transplantation of corrected (þA/þA) hematopoietic cells back into irradiated mice.
V. Homologous recombination with fiS and wild- type fiA allele via CRISPR- Cas9 genome editing

A) V, III, IV, I, II
B) V, III, I, IV, II
C) I, II, III, V, IV
D) II, III, V, I, IV
E) II, III, IV, V, I