Which of the Following Lists the Steps for CDNA Library

Multiple Choice

Which of the following lists the steps for cDNA library preparation in the correct order? I. Partially degrade the mRNA with RNase H
II. Isolate mRNA from a specific cell/ tissue with an oligo- dT primer that hybridizes to poly- A tail
III. Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV. Ligate cDNAs into a cloning vector
V. Create a single- stranded DNA molecule complementary to the mRNA using reverse transcriptase

A) II, V, IV, III, I
B) II, V, I, III, IV
C) I, II, III, IV, V
D) II, III, V, I, IV
E) V, III, I, IV, II