Question 1

IFE results are interpreted visually, by inspection of the bands produced. Characteristics of normal bands on IFE include all of the following except:

Accepted Answer

A)  bands are broad up and down the lane.
B)  narrow dense bands with sharp borders are present.
C)  gradual reduction in color intensity toward edges of the band.
D)  smooth reduction in intensity of band toward edges of the band. B

Question 2

A monoclonal gammopathy is

Accepted Answer

A)  frequently diagnosed using Immunofixation electrophoresis (IF
E)  .
B)  ruled out in a diagnosis of MGUS.
C)  ruled out in the case of negative results for IFE.
D)  ruled out in the case of negative results for serum and urine protein electrophoresis. A

Question 3

When comparing immunoelectrophoresis, IEP, and immunofixation electrophoresis, IFE, the following characteristics are true of IFE except that it is

Accepted Answer

A)  more complex.
B)  more sensitive.
C)  used for difficult to characterize anomalous proteins.
D)  challenging. D

Question 4

Advantages of Microchip capillary electrophoresis include

Accepted Answer

A)  high speed.
B)  reduced reagent consumption.
C)  integration analysis.
D)  All of the above.

Question 5

Electrophoresis is defined as

Accepted Answer

A)  migration of charged solutes in an electrical field.
B)  migration of charged particles in an electrical field.
C)  diffusion of charged particles in an electrical field.
D)  Both A and B.

Question 6

In addition to IFE, other techniques for the identification of M proteins include

Accepted Answer

A)  capillary zone electrophoresis.
B)  isotachophoresis.
C)  capillary isoelectric focusing.
D)  All of the above.

Question 7

Separation of proteins by electrophoresis is based on

Accepted Answer

A)  rate of migration of individual components in an electrical field.
B)  size of various molecules as they diffuse on cellulose acetate.
C)  the type of support medium such as cellulose acetate.
D)  All of the above.

Question 8

The most commonly identified M-protein in serum IFE is

Accepted Answer

A)  IgG.
B)  IgA.
C)  IgM.
D)  IgD.

Question 9

In the first phase of Immunofixation Electrophoresis (IFE), serum is separated according to

Accepted Answer

A)  electrophoretic mobility.
B)  diffusion coefficient.
C)  antibody specificity.
D)  All of the above.

Question 10

Immunofixation Electrophoresis (IFE) is a reliable and accurate method for detecting

Accepted Answer

A)  multiple myeloma.
B)  Waldenstrom's Macroglobulinemia.
C)  amyloid light chain amyloidosis.
D)  All the above.

Question 11

Normal results for serum IFE include

Accepted Answer

A)  darker IgG lane.
B)  lighter IgA lane.
C)  absent IgM lane.
D)  darker kappa lane compared to lambda lane.
E)  All the above.

Question 12

A polyclonal pattern observed in IFE results for serum or urine is considered

Accepted Answer

A)  specific for a monoclonal protein (M-protein) .
B)  nonspecific.
C)  to exhibit increased intensity of color in one or two lanes.
D)  to exclude the possibility of the presence of an M-protein.

Question 13

Suitable samples for Immunofixation Electrophoresis (IFE) include

Accepted Answer

A)  serum.
B)  urine.
C)  cerebrospinal fluid.
D)  other body fluids.
E)  All the above.

Exam 11: Electrophoresis Techniques