Migration of Proteins During Separation by SDS Polyacrylamide-Gel Electrophoresis (SDS-PAGE)

Short Answer

Migration of proteins during separation by SDS polyacrylamide-gel electrophoresis (SDS-PAGE) is generally related to the logarithm of their molecular weight, as depicted in the qualitative graph below. If a protein mixture containing proteins of evenly distributed molecular weights (e.g. 10, 20, 30, 40, etc.) is separated by SDS-PAGE, which of the following staining patterns would you expect to observe?