Exhibit 13A This Is a Map of the PBR322 Plasmid

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the Afl III site, the recombinant plasmid would lack which of the following properties?

A) It can replicate autonomously.
B) It will be resistant to the antibiotic tetracycline.
C) It will be resistant to the antibiotic ampicillin.
D) It will possess at lest two promoter sites.
E) All of these are true for the recombined plasmid.