Which of the Following Conditions Is Not a Requirement to Assure

Multiple Choice

Which of the following conditions is not a requirement to assure production of a protein when a plasmid is being designed to express a eukaryotic protein in a bacterium?

A) Introns must be deleted from the gene.
B) The cloning site must include an RNA polymerase promoter.
C) The mRNA product must contain a ribosome-binding site.
D) Differences in the genetic code between eukaryotes and prokaryotes must be accommodated.
E) All of these conditions must be met.