In Tandem Mass Spectrometry the Unique Second Phase Is Characterized

Multiple Choice

In tandem mass spectrometry the unique second phase is characterized by

A) the further isolation of the first phase samples by two-dimensional gel electrophoresis.
B) the purification of the first phase samples by column chromatography.
C) the addition of a collision based fragmentation step to the first phase sample to determine amino acid sequence.
D) running simultaneous samples to compare and confirm amino acid identity.