Question 1

Which statement regarding bacterial Rho is not true?

Accepted Answer

A)  Rho is a helicase. 
B)  Rho binds a specific C-rich sequence called a rut site. 
C)  Rho-dependent transcriptional termination depends on the ability of Rho to access the mRNA. 
D)  Rho rides along with RNA polymerase until a termination sequence is encountered.

Question 2

The TATA box (-10 or Pribnow box) has all the following characteristics except:

Accepted Answer

A)  It is both transcribed and translated. 
B)  It is upstream from most genes. 
C)  It is part of the binding site for the RNA polymerase. 
D)  It is part of a consensus (common) sequence of the promoter.

Question 3

Transcription proceeds in the

Accepted Answer

A)  5'$\rightarrow$ 3' and 3'$\rightarrow$ 5' direction 
B)  3'$\rightarrow$ 5' direction only 
C)  5'$\rightarrow$3' direction only 
D)  none of the above; 5 ' and 3 'are only important for DNA replication

Question 4

Which DNA strand is part of the DNA-RNA hybrid in the RNA polymerase "open complex," the template or nontemplate strand? Explain your answer.

Accepted Answer

The answer of Which DNA strand is part of the...

Question 5

In the absence of arabinose, the regulatory protein AraC

Accepted Answer

A)  forms a dimer, binds to two regulatory sequences near the promoter, and activatestranscription of the arabinose operon structural genes. 
B)  forms a dimer, binds to two distant sites, and forms a DNA loop that blocks access of RNApolymerase to the arabinose operon promoter. 
C)  binds to the arabinose operator and represses transcription of the arabinose operonstructural genes 
D)  binds to the arabinose operator and represses transcription of the arabinose operonstructural genes.

Question 6

Which statement is not true about the bacterial sigma factor?

Accepted Answer

A)  Sigma is a regulatory protein, which is required for the initiation of transcription. 
B)  Sigma has the ability to join ribonucleotides (NTPs) by phosphodiester bonds using DNA as a template. 
C)  Sigma properly orients the RNA polymerase complex for transcription at the gene start site. 
D)  After initiation is complete, sigma does not dissociate completely; some domains are displaced and mRNA synthesis continues.

Question 7

Rho-independent terminators have all of the following characteristics except:

Accepted Answer

A)  the consensus sequence is an inverted repeat 
B)  stem-loop structures can form within the mRNA 
C)  the repeat sequence is following by 7 to 8 uracil-containing nucleotides 
D)  sigma factor binding to the terminator triggers transcript release

Question 8

Draw diagrams of the lac operon that illustrate repressed transcription, basal transcription, and activated transcription.

Accepted Answer

The answer of Draw diagrams of the lac operon that...

Question 9

Transcription is the process of

Accepted Answer

A)  formation of an RNA transcript complementary to the DNA template strand. 
B)  formation of an RNA transcript complementary to the DNA non-template strand. 
C)  formation of RNA transcripts complementary to both strands of DNA. 
D)  formation of an RNA transcript complementary to the DNA sense (coding) strand.

Question 10

Transcription has a significant local effect on DNA structure (i.e., whether underwound or overwound). Discuss these structural changes and the enzymes that are required to resolve the situation.

Accepted Answer

The answer of Transcription has a significant local effect on...

Question 11

Diagram Jacob and Monod's operon model. Discuss some of the testable hypotheses put forth by this model.

Accepted Answer

The answer of Diagram Jacob and Monod's operon model. Discuss...

Question 12

A riboswitch regulates gene expression

Accepted Answer

A)  by influencing transcriptional initiation. 
B)  by influencing whether transcription continues to the end of a gene or operon. 
C)  by influencing whether a mRNA is translated. 
D)  B or C

Question 13

Design an experiment to demonstrate that the RNA polymerase holoenzyme binds DNA more tightly compared with the core polymerase. Show sample positive results.

Accepted Answer

The answer of Design an experiment to demonstrate that the...

Question 14

A particular sequence containing six base pairs is located in ten different organisms. The observed sequences are: 5′-ACGCAC-3′, ATACAC, GTGCAC, ACGCAC, ATACAC, ATGTAT, ATGCGC, ACGCAT, GTGCAT, and ATGCGC. What is the consensus sequence?

Accepted Answer

The answer of A particular sequence containing six base pairs...

Question 15

RNA polymerase binds to a specific region on DNA called a(n)

Accepted Answer

A)  origin 
B)  promoter 
C)  sigma factor 
D)  operon

Question 16

For bacterial operon induction, describe a specific example of the following: "DNA looping is a mechanism used in gene regulation."

Accepted Answer

The answer of For bacterial operon induction, describe a specific...

Question 17

The Jacob-Monod operon model

Accepted Answer

A)  led to the discovery of mRNA. 
B)  proposed the existence of a repressor protein. 
C)  both A and B 
D)  was later proved to be wrong.

Question 18

Alternative sigma factors A) induce specific groups of functionally related genes. B) replace the "standard" sigma factor $\sigma$⁷⁰ when cells are actively growing in optimal conditions. C) bind to riboswitches. D) are secreted by cells for quorum sensing.

Accepted Answer

The answer of Alternative sigma factors
A) induce specific groups of...

Question 19

Diagram the key features of the bacterial elongation complex. Include a rough sketch showing the direction of RNA synthesis and where a new phosphodiester bond forms.

Accepted Answer

The answer of Diagram the key features of the bacterial...

Question 20

Quorum sensing is a mechanism by which

Accepted Answer

A)  bacteria "count" the numbers of mRNA molecules that are made for a specific gene. 
B)  bacteria adjust the stability of an mRNA based on the presence of a metabolite. 
C)  bacteria change their transcriptional output in a manner that depends on their population density. 
D)  bacteria adjust total transcription levels in proportion to glucose levels.

Exam 10: Transcription in Bacteria

Which statement regarding bacterial Rho is not true?

The TATA box (-10 or Pribnow box) has all the following characteristics except:

Transcription proceeds in the

Which DNA strand is part of the DNA-RNA hybrid in the RNA polymerase "open complex," the template or nontemplate strand? Explain your answer.

In the absence of arabinose, the regulatory protein AraC

Which statement is not true about the bacterial sigma factor?

Rho-independent terminators have all of the following characteristics except:

Draw diagrams of the lac operon that illustrate repressed transcription, basal transcription, and activated transcription.

Transcription is the process of

Transcription has a significant local effect on DNA structure (i.e., whether underwound or overwound). Discuss these structural changes and the enzymes that are required to resolve the situation.

Diagram Jacob and Monod's operon model. Discuss some of the testable hypotheses put forth by this model.

A riboswitch regulates gene expression

Design an experiment to demonstrate that the RNA polymerase holoenzyme binds DNA more tightly compared with the core polymerase. Show sample positive results.

A particular sequence containing six base pairs is located in ten different organisms. The observed sequences are: 5′-ACGCAC-3′, ATACAC, GTGCAC, ACGCAC, ATACAC, ATGTAT, ATGCGC, ACGCAT, GTGCAT, and ATGCGC. What is the consensus sequence?

RNA polymerase binds to a specific region on DNA called a(n)

For bacterial operon induction, describe a specific example of the following: "DNA looping is a mechanism used in gene regulation."

The Jacob-Monod operon model

Alternative sigma factors A) induce specific groups of functionally related genes. B) replace the "standard" sigma factor $\sigma$ ⁷⁰ when cells are actively growing in optimal conditions. C) bind to riboswitches. D) are secreted by cells for quorum sensing.

Diagram the key features of the bacterial elongation complex. Include a rough sketch showing the direction of RNA synthesis and where a new phosphodiester bond forms.

Quorum sensing is a mechanism by which

Exam 10: Transcription in Bacteria

Which statement regarding bacterial Rho is not true?

The TATA box (-10 or Pribnow box) has all the following characteristics except:

Transcription proceeds in the

Which DNA strand is part of the DNA-RNA hybrid in the RNA polymerase "open complex," the template or nontemplate strand? Explain your answer.

In the absence of arabinose, the regulatory protein AraC

Which statement is not true about the bacterial sigma factor?

Rho-independent terminators have all of the following characteristics except:

Draw diagrams of the lac operon that illustrate repressed transcription, basal transcription, and activated transcription.

Transcription is the process of

Transcription has a significant local effect on DNA structure (i.e., whether underwound or overwound). Discuss these structural changes and the enzymes that are required to resolve the situation.

Diagram Jacob and Monod's operon model. Discuss some of the testable hypotheses put forth by this model.

A riboswitch regulates gene expression

Design an experiment to demonstrate that the RNA polymerase holoenzyme binds DNA more tightly compared with the core polymerase. Show sample positive results.

A particular sequence containing six base pairs is located in ten different organisms. The observed sequences are: 5′-ACGCAC-3′, ATACAC, GTGCAC, ACGCAC, ATACAC, ATGTAT, ATGCGC, ACGCAT, GTGCAT, and ATGCGC. What is the consensus sequence?

RNA polymerase binds to a specific region on DNA called a(n)

For bacterial operon induction, describe a specific example of the following: "DNA looping is a mechanism used in gene regulation."

The Jacob-Monod operon model

Alternative sigma factors A) induce specific groups of functionally related genes. B) replace the "standard" sigma factor σ\sigmaσ 70 when cells are actively growing in optimal conditions. C) bind to riboswitches. D) are secreted by cells for quorum sensing.

Diagram the key features of the bacterial elongation complex. Include a rough sketch showing the direction of RNA synthesis and where a new phosphodiester bond forms.

Quorum sensing is a mechanism by which

Alternative sigma factors A) induce specific groups of functionally related genes. B) replace the "standard" sigma factor $\sigma$ ⁷⁰ when cells are actively growing in optimal conditions. C) bind to riboswitches. D) are secreted by cells for quorum sensing.