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You Have Ligated a Foreign DNA Fragment into the EcoRI

Question 19

Multiple Choice

You have ligated a foreign DNA fragment into the EcoRI site in the multiple cloning site of pUC18. You then transform host E. coli with the ligation mixture. Some of the bacterial colonies growing on the nutrient agar plate that contains ampicillin and X-gal are white and some are blue. Bacterial colonies that are blue


A) contain recombinant plasmid DNA
B) contain nonrecombinant plasmid DNA
C) are untransformed bacteria
D) have hydrolyzed ampicillin to form a colored compound

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