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In a Single PCR Cycle Consisting of 30 Seconds at 95C

Question 39

Multiple Choice

In a single PCR cycle consisting of 30 seconds at 95C, 1 min at 55C, and 1 min at 72C, what is happening in the step run at 55C?


A) The DNA to be amplified is being denatured.
B) Primers are being denatured.
C) DNA polymerase is extending new DNA from the primers.
D) Primers are annealing to the DNA to be amplified.

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