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Dideoxy DNA Sequencing Is Based on Chain Termination with Dideoxynucleotides

Question 42

Multiple Choice

Dideoxy DNA sequencing is based on chain termination with dideoxynucleotides. Why are normal deoxynucleotides also included in the reaction?


A) to act as a primer for chain termination by the dideoxynucleotides
B) to provide a template for DNA polymerase
C) to allow production of a range of synthesis products of different lengths.
D) to provide a 2?-OH required for incorporation of the dideoxynucleotides

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