Essay
(a) Outline the steps that would be necessary to generate a cDNA library from all of the genes expressed by adenocarcinoma cells of a breast tumor. (b) How could tumor-specific transcripts be identified from this library? (Hint: think about how you could use non-cancerous breast epithelial cells as a comparison.)
Correct Answer:

Verified
(a)203-204
(1) Isolate mRNAs from cells ...View Answer
Unlock this answer now
Get Access to more Verified Answers free of charge
Correct Answer:
Verified
(1) Isolate mRNAs from cells ...
View Answer
Unlock this answer now
Get Access to more Verified Answers free of charge
Q7: When performing gel electrophoresis, why do molecular
Q8: Define the term "probe" and describe two
Q9: Describe the principle of gel filtration chromatography.
Q10: You have screened a library by nucleic
Q11: What are radioisotopes and why are they
Q13: The sequence read from a DNA sequencing
Q14: Type II restriction endonucleases are useful in
Q15: Describe X-ray crystallographic evidence for the mode
Q16: Diagram a strategy for screening a cDNA
Q17: The basic idea of using RFLPs as