Question 1

Which of the following is NOT a useful feature of a plasmid for cloning?

Accepted Answer

A) expressed sequence tag 
B) a cloning site 
C) a replicator (origin of replication) 
D) a selectable marker

Question 2

What would be the 9-residue primer used to amplify this sequence?
5'-ATCGACGTTACGCTACATAGCATAAGGCTT-3'

Accepted Answer

A) 5'-TAGCTGCAA-3' 
B) 5'-AAGCCTTAT-3' 
C) 5'-UAGCUGCAA-3' 
D) 5'-AAGCCUUAU-3'

Question 3

In the Southern hybridization procedure,after electrophoresis the gel is treated with NaOH and then neutralized before blotting.What is the primary function of the alkaline treatment?

Accepted Answer

A) to cleave the DNA into smaller fragments to permit greater efficiency of transfer 
B) to inactivate any restriction endonucleases that may be in the gel 
C) to denature the duplex DNA to single-stranded DNA (ssDN
A)  
D) to neutralize any acidic phosphate groups that might prevent hybridization

Question 4

What are linkers?

Accepted Answer

A) short sequences of DNA between the promoter and the gene 
B) short synthetic DNA duplexes consisting of a restriction site that is ligated onto DNA 
C) enzymes that link cloning sites in a sequence of nucleotides 
D) enzymes that incorporate foreign DNA into plasmids

Question 5

Reporter genes such as those for green fluorescent protein (GFP)are found on many commercial plasmids.What are these genes used for?

Accepted Answer

A) to determine if the plasmid is in the host 
B) to determine if a gene is present in a library 
C) to determine if a foreign protein is being expressed on a vector 
D) to determine the relative strength of a promoter sequence

Question 6

Which of the following is NOT correct for cDNA libraries?

Accepted Answer

A) Reverse transcriptase synthesizes a DNA strand complement of the mRNA templates. 
B) mRNA templates are isolated using oligo (d
A) -cellulose chromatography. 
C) Linkers are added and the cDNA is cloned into suitable vectors. 
D) The cDNA are copies from mRNA templates.

Question 7

Which of the following is NOT a part of the process associated with colony hybridization experiments?

Accepted Answer

A) A replica of the bacterial colonies is obtained on an absorbent disc. 
B) Autoradiography of the disc reveals probe-complementary DNA. 
C) Host bacteria with plasmid are plated and allowed to grow without media for days. 
D) The disc is treated with alkali.

Question 8

Which of the following is a property of shuttle vectors?

Accepted Answer

A) They contain promoters for the expression of the gene. 
B) They have origins of replication for 2 different cell types,usually bacteria and yeast. 
C) They are capable of incorporating very large DNA fragments. 
D) They contain more than 1 antibiotic-resistant gene.

Question 9

Which of the following is the appropriate source of the DNA polymerase included in the PCR reaction mixture?

Accepted Answer

A) E.coli 
B) bacteriophage T4 
C) Thermus aquaticus 
D) Drosophila melanogaster

Question 10

Which of the following is NOT a part of the procedure associated with the Southern blotting (hybridization)technique?

Accepted Answer

A) hybridization with radioactive probe 
B) agarose gel electrophoresis and visualize bands 
C) transfer (blot)to nitrocellulose filter 
D) digestion of DNA with DNA ligase

Exam 27: Recombinant Dna: Cloning and Creation of Chimeric Genes