Assume the Two E

Multiple Choice

Assume the two E.coli strains shown below are allowed to conjugate.
Hfr: pro⁺,arg⁺,his⁺,lys⁺,met⁺,ampicillin-sensitive
F-: pro⁻,arg⁻,his⁻,lys⁻,met⁻,ampicillin-resistant
What supplements would you add to glucose minimal salts agar to select for a new recombinant cell that is lys⁺,arg⁺,amp-resistant?

A) ampicillin,lysine,arginine
B) lysine,arginine
C) ampicillin,proline,histidine,methionine
D) proline,histidine,methionine
E) ampicillin,proline,histidine,lysine