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After PCR,the Amplification Control Has Failed to Yield a Product

Question 3

Multiple Choice

After PCR,the amplification control has failed to yield a product.What should you do?


A) Report out Indeterminate
B) Report out Negative because there was no amplification from the sample
C) Check the original DNA or RNA preparation.If it is adequate,repeat the amplification.If not,re-isolate the nucleic acid.
D) Repeat it using a higher concentration of amplification control in the reaction.

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