While Developing a New Hybridization-Based Molecular Test for Cryptosporidium,a Protozoan

Multiple Choice

While developing a new hybridization-based molecular test for Cryptosporidium,a protozoan parasite that causes diarrheal illness,a laboratory technician examines the hybridization ability with a closely related,but nontarget,protozoan and also gets a positive test result.In order to improve the stringency of the hybridization reaction for detection of Cryptosporidium only,the technician can do which of the following?

A) Decrease the salt concentration in the hybridization buffer
B) Increase the salt concentration in the hybridization buffer
C) Reduce the reaction temperature during hybridization
D) Remove destabilizing agents,such as formamide,from the hybridization buffer
E) None of the above