Why Must Double-Stranded DNA Probes Be Denatured When Used in Northern

Multiple Choice

Why must double-stranded DNA probes be denatured when used in northern blot experiments?

A) to ensure that they are properly radioactively labeled or tagged with a dye
B) to ensure that the DNA coding strand hybridizes to RNA on the membrane
C) to ensure that both DNA strands bind the single-stranded RNA on the membrane
D) all of the above
E) none of the above