You Are Growing a Population of Cells That Appear to Be

Multiple Choice

You are growing a population of cells that appear to be resistant to G418,a chemical that is normally toxic to cells.Upon further analysis of the cells' genome you find that it contains a genomic sequence that encodes the protein neomycin phosphotransferase.What would you do to test if this protein confers resistance to the G418 chemical?

A) determine if the cells are also making β-galactosidase
B) tag the protein with green fluorescent protein to see if it is degraded by G418
C) label a fragment of the gene to see where it is expressed
D) clone the gene into a G418-sensitive cell line,then treat these cells with G418