In the Large-Scale Production of a Particular Human Protein in E.coli

Multiple Choice

In the large-scale production of a particular human protein in E.coli cells,the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus.The purpose of this modification was to

A) facilitate transfer of the cDNA into the E.coli cells.
B) provide a promoter for the transcription of the cDNA in E.coli.
C) facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel atoms.
D) prevent degradation of the expressed protein by E.coli proteases.