Question 1

Humans can see objects as small as 0.1 mm.Leeuwenhoek's best microscope could magnify objects to 300X.Which of the following would be invisible using Leeuwenhoek's microscope?

Accepted Answer

A)   Poliovirus, 30 nm
B)   Red blood cell, 8  μ\muμ m
C)   Escherichia coli, 1  μ\muμ m X 4  μ\muμ m
D)   Pandora virus, 0.5 mm X 1 mm  A

Question 2

Who invented the first crude microscope by grinding glass?

Accepted Answer

A)   Lister
B)   Schultz and Schwann
C)   Leeuwenhoek
D)   Redi
E)   Pasteur C

Question 3

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

Accepted Answer

A)   A pure culture
B)   A contaminated medium
C)   A liquid medium
D)   A mixed culture
E)   A solid medium E

Question 4

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

A)   ocular
B)   condenser
C)   stage
D)   nosepiece

Question 5

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Accepted Answer

A)   incubated
B)   streaked
C)   spread
D)   poured

Question 6

What is the term for a culture made from one isolated colony?

Accepted Answer

A)   Axenic
B)   Mixed
C)   Pure
D)   Both axenic and mixed are correct.
E)   Both axenic and pure are correct.

Question 7

Some microbes are not capable of growing on artificial media as pure cultures.

Accepted Answer

A) True 
 B)False

Question 8

Comparing transmission electron microscopy with scanning electron microscopy,the following statement is true.

Accepted Answer

A)   Transmission EM is used for internal detail of cells and subcellular structures.
B)   Transmission EM gives detail of the external architecture of cells.
C)   Transmission EM requires dyes.
D)   Transmission EM can provide good images of bacteria but not viruses.

Question 9

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Accepted Answer

A)   enriched
B)   complex
C)   reducing
D)   synthetic

Question 10

Robert Koch and his colleagues first used agar in bacteriological media.Why was this ingredient an improvement on previous materials used to culture bacteria?

Accepted Answer

A)   Agar can convert from liquid to solid form, and back again, very easily.
B)   Agar is a complete source of nutrients for most bacteria.
C)   Bacteria growing on the solidified medium did not degrade or break down the agar.
D)   Agar was less expensive than the nutrients that Koch was previously using in the lab.

Question 11

The specimen preparation technique that is best for viewing cell motility is the ______.

Accepted Answer

A)   flagellar stain
B)   Gram stain
C)   negative stain
D)   hanging drop
E)   fixed, stained smear

Question 12

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Accepted Answer

A)   The microbiologist used too much inoculum.
B)   The culture medium was selective.
C)   The culture medium was differential.
D)   The culture was contaminated.
E)   The incubation temperature was incorrect.

Question 13

A common medium for culturing fastidious bacteria is ______.

Accepted Answer

A)   trypticase soy agar
B)   reducing medium
C)   mannitol salt agar
D)   blood agar
E)   MacConkey medium

Question 14

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

Accepted Answer

A) True 
 B)False

Question 15

What do the Gram stain,the acid-fast stain,and the endospore stain have in common?

Accepted Answer

A)   They use heat to force the dye into cell structures.
B)   The staining outcome varies with the charge of the cell wall.
C)   They are used on a wet mount of the specimen.
D)   They are differential stains.
E)   They use a negative stain technique.

Question 16

The wavelength of light used and the numerical aperture governs ______.

Accepted Answer

A)   resolution
B)   contrast
C)   illumination
D)   size of the field
E)   magnification

Question 17

Cells of Staphylococcus aureus are cocci measuring about 1 $\mu$m in diameter.In a textbook image of S. aureus,each cell measures about 1 cm.The magnification of the image on the page is ______.

Accepted Answer

A)  10,000X
B)  1,000X
C)  100X
D)  0.01X
E)  0.001XF) 0.0001XG) This is impossible to calculate without more information.

Question 18

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea.Although it can be isolated from the vagina,there are numerous normal biota in that location as well.To generate a pure culture of this pathogenic bacterium,the best choice is a(n)______.

Accepted Answer

A)   differential medium
B)   enrichment medium
C)   selective medium
D)   liquid medium

Question 19

Which type of microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A)   Bright-field
B)   Fluorescence
C)   Electron
D)   Dark-field
E)   Phase-contrast

Question 20

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

Accepted Answer

A)   reducing
B)   enriched
C)   complex
D)   defined

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Humans can see objects as small as 0.1 mm.Leeuwenhoek's best microscope could magnify objects to 300X.Which of the following would be invisible using Leeuwenhoek's microscope?

Who invented the first crude microscope by grinding glass?

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

The _____ of the microscope holds and allows selection of the objective lenses.

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

What is the term for a culture made from one isolated colony?

Some microbes are not capable of growing on artificial media as pure cultures.

Comparing transmission electron microscopy with scanning electron microscopy,the following statement is true.

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Robert Koch and his colleagues first used agar in bacteriological media.Why was this ingredient an improvement on previous materials used to culture bacteria?

The specimen preparation technique that is best for viewing cell motility is the ______.

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

A common medium for culturing fastidious bacteria is ______.

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

What do the Gram stain,the acid-fast stain,and the endospore stain have in common?

The wavelength of light used and the numerical aperture governs ______.

Cells of Staphylococcus aureus are cocci measuring about 1 $\mu$ m in diameter.In a textbook image of S. aureus,each cell measures about 1 cm.The magnification of the image on the page is ______.

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea.Although it can be isolated from the vagina,there are numerous normal biota in that location as well.To generate a pure culture of this pathogenic bacterium,the best choice is a(n) ______.

Which type of microscope achieves the greatest resolution and highest magnification?

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Humans can see objects as small as 0.1 mm.Leeuwenhoek's best microscope could magnify objects to 300X.Which of the following would be invisible using Leeuwenhoek's microscope?

Who invented the first crude microscope by grinding glass?

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

The _____ of the microscope holds and allows selection of the objective lenses.

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

What is the term for a culture made from one isolated colony?

Some microbes are not capable of growing on artificial media as pure cultures.

Comparing transmission electron microscopy with scanning electron microscopy,the following statement is true.

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Robert Koch and his colleagues first used agar in bacteriological media.Why was this ingredient an improvement on previous materials used to culture bacteria?

The specimen preparation technique that is best for viewing cell motility is the ______.

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

A common medium for culturing fastidious bacteria is ______.

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

What do the Gram stain,the acid-fast stain,and the endospore stain have in common?

The wavelength of light used and the numerical aperture governs ______.

Cells of Staphylococcus aureus are cocci measuring about 1 μ\muμ m in diameter.In a textbook image of S. aureus,each cell measures about 1 cm.The magnification of the image on the page is ______.

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea.Although it can be isolated from the vagina,there are numerous normal biota in that location as well.To generate a pure culture of this pathogenic bacterium,the best choice is a(n) ______.

Which type of microscope achieves the greatest resolution and highest magnification?

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

Cells of Staphylococcus aureus are cocci measuring about 1 $\mu$ m in diameter.In a textbook image of S. aureus,each cell measures about 1 cm.The magnification of the image on the page is ______.