In Sanger Sequencing, What Causes DNA Synthesis to Terminate at a Specific

Multiple Choice

In Sanger sequencing, what causes DNA synthesis to terminate at a specific base?

A) chemicals that cleave DNA after particular bases
B) fluorescent chemicals
C) nucleotide triphosphates that lack a base
D) nucleotide triphosphates that lack a hydroxyl at the 3' position
E) nucleosides that lack a 5' phosphate