Question 1

Candidate open reading frames of a genome are identified by searching for

Accepted Answer

A) a start codon "in frame" with a stop codon. 
B) a start codon. 
C) introns and exons. 
D) a promoter. 
E) All of these

Question 2

In a physical map,distances are measured in

Accepted Answer

A) recombination frequencies. 
B) number of SNPs. 
C) base pairs. 
D) probabilities. 
E) microns.

Question 3

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking ampS1U1P1RS1S1P0, however.
-What problem does this result in?

Accepted Answer

The answer of A pBluescript II cloning vector is used...

Question 4

A linear strand of DNA with two restriction enzyme cut sites will yield three fragments on digestion.

Accepted Answer

A) True 
 B)False

Question 5

Discuss the relative roles of descriptive and hypothesis-driven science in genomics.

Accepted Answer

The answer of Discuss the relative roles of descriptive and...

Question 6

A DNA copy of an mRNA molecule is called

Accepted Answer

A) dDNA. 
B) rDNA. 
C) mDNA. 
D) shDNA. 
E) cDNA.

Question 7

Bacteria protect their own DNA from restriction enzyme damage by adding ________ groups to certain nucleotides.

Accepted Answer

A) methyl 
B) hydroxyl 
C) phosphate 
D) amino 
E) carboxyl

Question 8

The human genome consists mostly of noncoding DNA.

Accepted Answer

A) True 
 B)False

Question 9

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking ampS1U1P1RS1S1P0, however.
-Can you think of an alternative means of identifying successful bacterial transformants in this experiment?

Accepted Answer

The answer of A pBluescript II cloning vector is used...

Question 10

The restriction enzyme SmaI cuts DNA at the 6-base recognition sequence 5'-CCCGGG-3'.Calculate the frequency of occurrence of this sequence.(Assume a 50% GC content.)

Accepted Answer

The answer of The restriction enzyme SmaI cuts DNA at...

Exam 8: Genomics: The Mapping and Sequencing of Genomes

Candidate open reading frames of a genome are identified by searching for

In a physical map,distances are measured in

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking amp^R, however. -What problem does this result in?

A linear strand of DNA with two restriction enzyme cut sites will yield three fragments on digestion.

Discuss the relative roles of descriptive and hypothesis-driven science in genomics.

A DNA copy of an mRNA molecule is called

Bacteria protect their own DNA from restriction enzyme damage by adding ________ groups to certain nucleotides.

The human genome consists mostly of noncoding DNA.

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking amp^R, however. -Can you think of an alternative means of identifying successful bacterial transformants in this experiment?

The restriction enzyme SmaI cuts DNA at the 6-base recognition sequence 5'-CCCGGG-3'.Calculate the frequency of occurrence of this sequence.(Assume a 50% GC content.)

Exam 8: Genomics: The Mapping and Sequencing of Genomes

Candidate open reading frames of a genome are identified by searching for

In a physical map,distances are measured in

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking ampR, however. -What problem does this result in?

A linear strand of DNA with two restriction enzyme cut sites will yield three fragments on digestion.

Discuss the relative roles of descriptive and hypothesis-driven science in genomics.

A DNA copy of an mRNA molecule is called

Bacteria protect their own DNA from restriction enzyme damage by adding ________ groups to certain nucleotides.

The human genome consists mostly of noncoding DNA.

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking ampR, however. -Can you think of an alternative means of identifying successful bacterial transformants in this experiment?

The restriction enzyme SmaI cuts DNA at the 6-base recognition sequence 5'-CCCGGG-3'.Calculate the frequency of occurrence of this sequence.(Assume a 50% GC content.)

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking amp^R, however. -What problem does this result in?

A pBluescript II cloning vector is used to introduce a gene of interest into a bacterial strain. The vector is lacking amp^R, however. -Can you think of an alternative means of identifying successful bacterial transformants in this experiment?