Question 1

Knockout technology uses a natural process.It is

Accepted Answer

A)  DNA repair. 
B)  DNA amplification. 
C)  DNA replication. 
D)  homologous recombination. 
E)  transcription.

Question 2

The polymerase chain reaction is used to

Accepted Answer

A)  create millions of copies of an interesting piece of DNA. 
B)  speed the rate of DNA replication in cells. 
C)  make more copies of DNA polymerase. 
D)  copy protein into RNA. 
E)  make RNA in the cell nucleus.

Question 3

Bt toxin-producing plants are resistant to

Accepted Answer

A)  fungal pathogens. 
B)  bacterial pathogens. 
C)  herbicides. 
D)  insect pests. 
E)  bacteriophage.

Question 4

The first drug produced using recombinant DNA technology was

Accepted Answer

A)  insulin. 
B)  streptokinase. 
C)  tPA. 
D)  EPO. 
E)  Viagra.

Question 5

Which approach could be used to investigate distinctions between a muscle cell and a bone cell?

Accepted Answer

A)  gene expression profiling 
B)  DNA variation screening 
C)  microarray comparative genomic hybridization 
D)  transcription-mediated amplification 
E)  PCR

Question 6

Which of the following are required for PCR?

Accepted Answer

A)  knowing a protein's amino acid sequence 
B)  primers complementary to the entire target DNA sequence 
C)  the four nucleotides in the proportions of the target DNA sequence 
D)  Taq2 RNA polymerase 
E)  knowing part of the target DNA sequence that you want to amplify

Question 7

A multicellular organism that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is

Accepted Answer

A)  transverted. 
B)  translocated. 
C)  transgenic. 
D)  transformed. 
E)  transliterate.

Question 8

A genomic library consists of

Accepted Answer

A)  a list of human genes whose functions have been identified and that have been mapped to a chromosomal locus. 
B)  a list of human genes, including SNPs and mutations. 
C)  recombinant bacteria that collectively include several copies of a genome. 
D)  a facility that stores books about genetics. 
E)  a set of DNA probes corresponding to a genome.

Question 9

Restriction enzymes are useful in creating recombinant DNA molecules because they

Accepted Answer

A)  generate "sticky ends" by cutting at the same sequence on both strands. 
B)  are found only in human cells, where they naturally mend broken DNA strands. 
C)  are only attracted to and act on DNA sequences from different species. 
D)  are used to encapsulate and transport foreign DNA into cells of the target organism. 
E)  stimulate DNA amplification so that the desired cells or their products can be scaled up.

Question 10

A molecule that consists of a piece of DNA from one organism combined with the DNA from a member of another species is called

Accepted Answer

A)  restricted DNA. 
B)  recombinant DNA. 
C)  transgenic DNA. 
D)  bioengineered DNA. 
E)  mutant DNA.

Question 11

The requirements for patenting of an invention involving DNA in the U.S.are that it

Accepted Answer

A)  be new, useful, and not obvious to an expert in the field. 
B)  be obvious, but no one had previously been able to accomplish it. 
C)  be helpful and affordable. 
D)  be new, useful, and predicted by experts in the field. 
E)  combine DNA from different species in the same cell.

Question 12

Which technique amplifies viral RNA in a patient's blood specimen?

Accepted Answer

A)  antisense engineering 
B)  a knockout gene 
C)  a knockin gene 
D)  transcription-mediated amplification 
E)  traditional PCR

Question 13

Bacteriophages can be used as vectors in recombinant DNA experiments because they

Accepted Answer

A)  are small and made of double-stranded DNA. 
B)  are circular and easily imported into bacteria or yeast. 
C)  insert their genetic material into bacteria. 
D)  are resistant to protective restriction systems. 
E)  infect human and other animal cells.

Question 14

A gene expression microarray has

Accepted Answer

A)  an entire genome of DNA, cut into hundreds of pieces, attached to a small plastic or glass square. 
B)  short pieces of DNA of known sequence attached to a small plastic or glass square. 
C)  several human genes sent into bacterial cells, and the cells allowed to divide. 
D)  short pieces of RNA of known sequence attached to a small plastic or glass square. 
E)  amino acids attached to a small plastic or glass square.

Question 15

The use of short,synthetic,double-stranded RNAs to intentionally destroy specific mRNAs is called ________ technology.

Accepted Answer

A)  knockup 
B)  knockdown 
C)  PCR 
D)  bioremediation 
E)  nonsense

Question 16

A naturally-occurring,small,circle of DNA used as a vector to transmit DNA is a

Accepted Answer

A)  plasmid. 
B)  prion. 
C)  liposome. 
D)  lipofectin. 
E)  ring chromosome.

Question 17

Transgenic organisms carry the transgene in

Accepted Answer

A)  every cell. 
B)  gametes only. 
C)  somatic cells only. 
D)  the cell in which it was originally introduced. 
E)  viral cultures.

Exam 19: Genetic Technologies: Amplifying, Modifying, and Monitoring DNA