Which of the Following Explains Why It Is Necessary to Use

Multiple Choice

Which of the following explains why it is necessary to use safranin in the Gram stain?

A) Gram-positive bacteria do not have a strong affinity for crystal violet, but do bind well to safranin
B) Gram-negative bacteria are rendered colorless after the decolorizer is added, and need to be counterstained to be visible under the microscope
C) Safranin is the mordant needed to set the crystal violet color and make it permanent
D) Gram-variable cells do not bind to crystal violet and need to be counterstained to be visible under the microscope
E) None of the choices are true