Essay
A linear molecule of DNA 9 kb in length is isolated and mapped using restriction endonucleases EcoRI and HindIII.The fragments generated by the single and double digests are shown below.The numbers refer to the length of fragments in kilobases.
EcoRI: 7 kb,2 kb
HindIII: 5 kb,4 kb
EcoRI + HindIII: 4 kb,3 kb,2 kb
SalI: 8.5 kb,0.5 kb
EcoRI + SalI: 7 kb,1.5 kb,0.5 kb
Construct a restriction map of this linear fragment of DNA.
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