The Drawing Below Depicts a Restriction Map of a Segment

Essay

The drawing below depicts a restriction map of a segment of a human chromosome.The cut sites shown (1 through 6)are known to be polymorphic and can be present or absent depending on the genetic makeup of a specific individual's chromosomes.E identifies EcoRI sites,and P identifies Pst1 sites.The line below the map identifies the region of homology between the DNA and a probe used in Southern blot analyses to characterize this region. a)Individual 1 has restriction sites 1 through 6 (all)on both copies of this chromosome.If DNA from this individual was digested with Pst I,what are the sizes of the fragments that would be detected via Southern blot using the identified probe?
b)DNA from Individual 1 is digested with both EcoRI and PstI and then analyzed by Southern blot.What bands are revealed?
c)Individual 2 is homozygous for a polymorphism that eliminates site 3.If DNA from this individual was digested with both PstI and EcoRI,what are the sizes of the fragments that would be detected via Southern blot?
d)Individual 3 has not previously been characterized over this chromosomal locus.The research team digests the DNA with PstI and EcoRI and generates the following banding pattern.What is the likely genotype of this individual?