Question 1

Reverse transcriptase generates cDNA from a ـــــــــــــــــــtemplate.

Accepted Answer

A) DNA 
B) transfer RNA 
C) messenger RNA 
D) cDNA C

Question 2

Restriction enzymesــــــــــــــــــــ .

Accepted Answer

A) generate either blunt or sticky ends by cutting the DNA on each strand. 
B) are nucleases. 
C) recognize specific sequences and cleave the DNA at the recognition site or somewhere in the vicinity. 
D) are all of the above. D

Question 3

Which process enables recombinant DNA to be introduced into mammalian cell lines?

Accepted Answer

A) Transfection 
B) Transduction 
C) Transformation 
D) Translocation A

Question 4

The purpose of the centromere sequence in YACs is toــــــــــــــــــــ .

Accepted Answer

A) provide a multiple cloning site 
B) allow correct partitioning of the plasmid in yeast 
C) allow the vector to replicate in the yeast 
D) select for the presence of the vector in the host

Question 5

A collection of cloned DNA fragments is called aـــــــــــــــــــــ .

Accepted Answer

A) consortium 
B) chromosomal database 
C) chromosome library 
D) gene library

Question 6

Cells methylate their own DNA toــــــــــــــــــــ .

Accepted Answer

A) mark the DNA for degradation 
B) prevent replication 
C) protect the DNA from the cell's own restriction enzymes 
D) stabilize the DNA double helix

Question 7

What types of genes are found on plasmids and often used as selectable markers?

Accepted Answer

A) antibiotic resistance genes 
B) multiple cloning sites 
C) origins of replication 
D) methylated sequences

Question 8

The enzyme that links the ends of two DNA molecules is calledــــــــــــــــــــــ .

Accepted Answer

A) reverse transcriptase 
B) DNA polymerase 
C) DNA ligase 
D) DNA helicase

Question 9

Which of the following cloning vectors carries the largest insert relative to the others in the answer choices?

Accepted Answer

A) BACs 
B) cosmids 
C) PACs 
D) plasmids

Question 10

Yeast artificial chromosomes must haveـــــــــــــ and ــــــــــــــــin order to function within eukaryotes.

Accepted Answer

A) origin of replication;internal resolution sites 
B) telomere sequences;centromere sequences 
C) multiple cloning sites;genes for nuclear proteins 
D) multiple promoters;genes for antibiotic resistance

Question 11

Why must a shuttle vector possess two origins of replication instead of one?

Accepted Answer

A) Shuttle vectors must replicate in multiple hosts with each host requiring a different origin. 
B) Shuttle vectors must be able to replicate from two different origins of replication depending upon the specific growth conditions. 
C) Shuttle vectors only have one origin of replication,not two. 
D) Shuttle vectors use one origin for replication and the other origin for shuttling recombinant DNA into new hosts.

Exam 7: Recombinant DNA Technology