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A Southern Blot Procedure Has Been Performed, but the Molecular

Question 1

Multiple Choice

A Southern blot procedure has been performed, but the molecular weight markers are the only bands seen on the gel. Apparently, the probe did not bind to its target on the membrane. How might the stringency be adjusted so that the probe will bind?


A) Decrease the incubation temperature.
B) Decrease the salt concentration.
C) Increase the formamide concentration.
D) Increase the incubation temperature.

Correct Answer:

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