Multiple Choice
A hybridization assay is run under the following conditions: 40% formamide with 0.5X SSC at 70oC. When the bands were visualized, there were many more bands than expected in all wells on the membrane. Which of the following changes should be made to the hybridization conditions to get results that are closer to those expected?
A) Decrease formamide
B) Increase temperature
C) Increase SSC concentration
D) Decrease temperature
Correct Answer:
Verified
Correct Answer:
Verified
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