The Enzyme Hexokinase Catalyzes the Reaction Between ATP and Glucose

Multiple Choice

The enzyme hexokinase catalyzes the reaction between ATP and glucose to form glucose 6-phosphate and ADP.In order to determine the efficiency of hexokinase, you wish to conduct an experiment to calculate its turnover number.To do so, you would set up

A) a reaction with a maximum amount of hexokinase at optimal pH and temperature.
B) a reaction with a known amount of enzyme and saturating levels of glucose 6-phosphate at optimal pH and temperature.
C) a reaction with a known amount of enzyme and saturating levels of glucose and ATP at optimal pH and temperature.
D) multiple reactions at varying pH levels but with a constant amount of glucose, a constant temperature, and a known amount of enzyme.
E) multiple reactions with varying temperatures but a constant amount of glucose, constant pH levels, and a known amount of enzyme.