The Sequence 5ʹ-TCACGT-3ʹ Is Not a Restriction Enzyme Palindromic Recognition

Multiple Choice

The sequence 5ʹ-TCACGT-3ʹ is not a restriction enzyme palindromic recognition sequence.What mutational change would most likely make it into one?

A) Changing T to C at the 5ʹ end
B) Changing T to A at the 5ʹ end
C) Changing T to A at the 3ʹ end
D) Changing T to G at the 3ʹ end
E) No single point mutation can change this sequence into a recognition sequence.