A Restriction Enzyme Recognizes the Sequence 5ʹ-CTGCAG-3ʹ and Makes Cuts

Multiple Choice

A restriction enzyme recognizes the sequence 5ʹ-CTGCAG-3ʹ and makes cuts between G and C.Which statement is most likely true?

A) Sticky ends should arise because the enzyme cuts in the middle of the sequence.
B) Sticky ends should arise because the restriction site is less than eight nucleotides long.
C) Sticky ends should arise because the restriction site is more than four nucleotides long.
D) Blunt ends should arise because the enzyme cuts once in the middle of the sequence.
E) Blunt ends should arise because the restriction site is more than four nucleotides long