EcoRI Makes Staggered Cuts When It Cleaves DNA, Creating Single-Stranded

Multiple Choice

EcoRI makes staggered cuts when it cleaves DNA, creating single-stranded tails called "sticky ends." These ends can bind to complementary bases.Assuming that concentration of the cut DNA strands is not rate limiting, in order for the complementary ends to base pair, which condition is necessary?

A) The presence of specific helicases
B) Sufficiently high temperature
C) The presence of methyl groups at the end of each DNA strand
D) Sufficiently low temperature
E) Phosphodiester bonds between the complementary DNA strands