It Is Suspected That the Gene DEFT Regulates the Expression

Multiple Choice

It is suspected that the gene DEFT regulates the expression of many other genes.How could one test this?

A) Knock out different genes using CRISPR, then see how DEFT is expressed using microarrays.
B) Knock out different genes using CRISPR, then see how DEFT is expressed using RNAi.
C) Use CRISPR to knock DEFT out, then use microarrays to examine how genes are expressed with and without DEFT expression.
D) Use CRISPR to knock DEFT out, then use RNAi to examine how genes are expressed with and without DEFT expression.
E) Use microarrays to knock out DEFT, then use RT-PCR to see how genes are expressed with and without DEFT expression.