You Are Performing a Recombinant DNA Experiment Using a Restriction

Multiple Choice

You are performing a recombinant DNA experiment using a restriction enzyme that makes staggered cuts and using the GFP gene as a reporter.The plasmid DNA gets cut properly, but the different pieces do not rejoin properly.Most likely, you have a defective

A) CAS9 protein.
B) restriction enzyme.
C) DNA ligase.
D) GFP.
E) replicon.