Variation at a Specific Gene Is Known to Affect Burrowing

Multiple Choice

Variation at a specific gene is known to affect burrowing behavior in a rodent, but it is not clear which of the nucleotides are responsible for the changes.Which of these techniques would be most relevant for revealing which nucleotides are responsible?

A) Mutagenesis via RNA interference
B) Mutagenesis via CRISPR
C) Gene expression analysis via CRISPR
D) Gene expression analysis via microarrays
E) Knockout studies via CRISPR