Which of the Following Would Not Be Required for the Purpose

Multiple Choice

Which of the following would not be required for the purpose of determining the sequence of a 400-base-pair DNA fragment by the standard ddNTP sequencing method?

A) The four dNTPs (dATP, dCTP, dGTP, dTTP)
B) DNA polymerase
C) A short, artificially synthesized primer
D) A bacterial artificial chromosome
E) All of the above would be required.