The Original Development of PCR Did Not Use the Taq

Multiple Choice

The original development of PCR did not use the Taq polymerase. What is the reason that the Taq polymerase is currently used?

A) the DNA melting step at 95°C would cause denaturation of most DNA polymerases from other organisms
B) the Taq polymerase is one of the most rapid DNA polymerase enzymes known and is therefore extremely useful for PCR
C) the original method of PCR required that DNA polymerase be added prior to each round of elongation, something that is not conducive to automation
D) the Taq polymerase is the only DNA polymerase that will allow production of 2ⁿ amounts of DNA (where n=number of cycles)
E) both A and C are corect