Explain How the CRISPR/Cas9 System Can Be Used to Edit

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The Answer is A nucleotide sequence in any genome can be edited with an engineered version of the CRISPR/Cas9 system found in the bacterium Streptococcus pyogenes. S. pyogenes Cas9 is an RNA-guided endonuclease that catalyzes cleavage of both strands of a dsDNA at a site determined by base-pairing between the Cas9-associated guide RNA (gRNA) and the target DNA nucleotide sequence. Since a gRNA of any desired nucleotide sequence is easily made, any DNA sequence is a legitimate target for Cas9 endonucleolytic cleavage. DNA repair systems such as nonhomologous end joining (NHEJ) and homology-directed repair (HDR) will repair the double-stranded breaks, and the genes can be screened for the hoped-for changes in nucleotide sequence.​

Explain How the CRISPR/Cas9 System Can Be Used to Edit

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Explain How the CRISPR/Cas9 System Can Be Used to Edit

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Correct Answer:VerifiedShow Answer

Correct Answer:
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