One Early Study on DNA Vaccines Examined the CD8 T

Multiple Choice

One early study on DNA vaccines examined the CD8 T cell response to the plasmid-encoded antigenic protein following subcutaneous immunization of mice with the plasmid DNA. This study used bone marrow chimeras to distinguish the MHC class I alleles expressed on bone marrow-derived cells from those expressed on all other mouse cells and tissues. When analyzed, the data showed that antigen-specific CD8 T cell responses were restricted to the MHC class I alleles derived from the donor bone marrow. These findings indicate that a key step in DNA vaccination is:

A) The uptake of the plasmid DNA by antigen-presenting cells in the skin
B) The expression and secretion of the plasmid DNA-encoded antigenic protein by skin keratinocytes
C) The stimulation of keratinocytes to produce inflammatory cytokines in response to the plasmid DNA-encoded antigenic protein
D) The presentation of peptides derived from the antigenic protein on MHC class I molecules of skin-resident dendritic cells
E) The trafficking of the plasmid DNA-encoded antigenic protein to the draining lymph nodes of the skin for uptake by phagocytic cells