Fatal Anaphylaxis Can Be Induced in Mice by Sensitizing the Mice

Multiple Choice

Fatal anaphylaxis can be induced in mice by sensitizing the mice to penicillin V (Pen V) . To elicit this response, Pen V is conjugated to a protein, such as chicken ovalbumin (OVA) , and mice are immunized with this conjugate by intraperitoneal (IP) injection in a T_H2-inducing adjuvant. Fourteen days later, mice are injected intravenously (IV) with Pen V conjugated to a different protein, bovine serum albumin (BSA) , and examined 20 minutes later. In addition, a second set of mice received anti-IL-4 antibody injections on days 0, 2, and 4 of the sensitization phase of the response. The results in Table Q16) A were observed; (data are shown as the ratio of dead mice to total mice for each condition) :as above. Twenty-four days later, serum from these mice was isolated and injected into a set of naive (unimmunized) recipient mice. Recipient mice were then sensitized by a single IP injection of Pen V-OVA in adjuvant, as above, and then 24 hours later, were given an IV injection of Pen V-BSA or BSA alone. In addition, one group of mice received serum that was depleted of specific antibodies prior to transfer into recipients. These mice develop a slightly milder disease, characterized by severe shock rather than death. Data are shown in Table Q16) B as the ratio of mice exhibiting severe shock to total mice for each condition/ Table Q16) B
Which antibodies were depleted from the serum in the final experiment shown above:

A) Antibodies specific to BSA
B) Antibodies specific to OVA
C) All IgG antibodies
D) Antibodies to IL-4
E) All IgE antibodies