Which of the Following Is a Disadvantage of Map-Based Sequencing

Multiple Choice

Which of the following is a disadvantage of map-based sequencing over shotgun sequencing?

A) Map-based sequencing minimizes the amount of repeat sequencing in which the same region is sequenced several times.
B) Map-based sequencing yields well-characterized physical maps as well as actual sequences.
C) The relationship of overlapping fragments to sequences is known before sequencing begins.
D) Map-based sequencing is more time-consuming because extensive mapping is required before sequencing starts.
E) Restriction patterns can be used to identify overlapping clones.