Why Are Scientists Unable to Grow the Majority of Bacteria

Multiple Choice

Why are scientists unable to grow the majority of Bacteria and Archaea in pure culture in a laboratory setting?

A) Most microorganisms reside in extreme environments that are toxic to human life, so samples cannot be collected for cultivation of the microbial inhabitants in the laboratory.
B) Many species have metabolic requirements that are difficult to fulfill in the laboratory.
C) DNA sequencing technology was not sufficiently advanced to give scientists the ability to identify prokaryotic species in nature, a requirement for growing microbes in the laboratory.
D) Cells with unusual morphological characteristics cannot be grown in the laboratory.