Which of the Following Make(s) Sequencing by the Sanger Chain-Termination

Multiple Choice

Which of the following make(s) sequencing by the Sanger chain-termination method possible?

A) New nucleotides are added only to the 3' end of a growing DNA strand.
B) A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated.
C) Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule.
D) Duplex nucleic acid molecules can be separated by size by means of electrophoresis.
E) Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper.