Using PCR, a Student Hopes to Amplify a Sequence of Known

Multiple Choice

Using PCR, a student hopes to amplify a sequence of known DNA from a genomic DNA sample. The two primers are both designed to pair with sequences that have a 40% GC content. Using a particular set of denaturation temperatures, annealing temperatures, and extension temperatures, the experiment produces not the expected single DNA fragment of a single known size, but a set of fragments of different sizes. Which of the following could account for these results?

A) The sequences of the primers were not specific enough.
B) The annealing temperature was too high.
C) The denaturation temperature was too high.
D) The extension temperature was too low.