Question 1

Figure Q3-6 is from an experiment that helped show that neurotransmitter release is triggered by presynaptic Ca²⁺. In panel A, the presynaptic membrane potential was clamped at -25mV. Figure Q3-6 A. How did they know Ca²⁺ entered the presynaptic neuron? B. What happened in the postsynaptic neuron? C. In panel B, the presynaptic membrane potential was clamped at -50mV. Why was there no net current during the presynaptic depolarization? D. Why was there a current after the presynaptic potential was clamped back to -70mV? E. What happened to the delay in postsynaptic current with the +50mV presynaptic depolarization?

Accepted Answer

The answer of Figure Q3-6 is from an experiment that...

Question 2

You take a 'normal' oocyte and apply ACh by iontophoresis to the oocyte and record the resulting current response with voltage clamp
A. What will the resultant current be? Explain.
B. You then inject the oocyte with mRNAs for AChR subunits and apply ACh. What will happen? Explain your answer.

Accepted Answer

The answer of You take a 'normal' oocyte and apply...

Question 3

Figure Q3-4 documents the quantal release of neurotransmitter. Figure Q3-4 A. The investigators stimulated the nerve in low-Ca²⁺ and high-Mg²⁺ saline so that they had many failures with nerve stimulation. Why was the presence of failures so important? B. In the top trace, there were three motor nerve stimulations that occurred at the arrow. Why is the flat trace flat and, based on what we know now, what is the reason that the other two traces are multiples of each other? C. The investigators compared the evoked EPPs in low Ca²⁺/high Mg²⁺ saline to spontaneous EPPs (mEPP). What did they find (see figures on the right) and what did they conclude? D. Based on the findings in this figure, what did they conclude about transmitter release in an evoked EPP in normal saline?

Accepted Answer

The answer of Figure Q3-4 documents the quantal release of...

Question 4

Match between columns
                        Premises: Higher probability of release
Strings of amino acids
Derived from amino acids
Synthesized in the cell body
Synaptic vesicles
Dense core vesicles
Synthesized in the axon terminal

Accepted Answer

The Answer of neuropeptides. and small molecule transmitters is...

Question 5

Fill in the missing words in the following sentence.
Glutamate is an amino acid. The neurotransmitter, _______, is derived from glutamate by the enzyme _______.

Accepted Answer

The answer of Fill in the missing words in the...

Question 6

In adult neurons, there is a higher concentration of chloride outside the cell than inside the cell. During development intracellular chloride concentrations are much higher such that GABA causes excitation of the postsynaptic neuron. Why could changing the Cl- concentration result in excitation?

Accepted Answer

A)  ECl is now > action potential threshold. 
B)  ECl is now 2+ conductance. 
D)  Higher concentrations of Cl- modulate the GABAR single channel conductance.

Question 7

How many ACh binding sites are there on each AChR?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
E)  5

Question 8

The canonical model for neurotransmitter release is that there is synaptic input to a dendrite which, if large enough depolarizes the voltage across the membrane and generates an action potential at the axon hillock, which travels to the axon terminal and causes the release of neurotransmitter.
Based on the concepts in this chapter, discuss how the properties below alter this model.
A. Active properties of dendrites
B. Inhibition along the dendrite
C. Presynaptic inhibition
D. Summation?

Accepted Answer

The answer of The canonical model for neurotransmitter release is...

Question 9

Neurotransmitters can result in long-term changes in the physiological state of a neuron. One way this can happen is through activation of transcription factors, like CREB. How can neurotransmitters effect gene expression in neurons? Be as specific as possible.

Accepted Answer

The answer of Neurotransmitters can result in long-term changes in...

Question 10

Otto Loewi won a Noble Prize in 1936 for showing that nerves release chemical transmitters. For one of his experiments he showed that stimulating the vagus nerve caused the heartbeat to slow down. He then collected the 'releasate' from nerve stimulation and put it on another heart, in which the heartbeat slowed as well. The substance that was release was later identified as ACh. What do we know now about the mechanism by which ACh decreases heart rate? Fill in the blanks in the following sentence.
ACh binds to the _____ cholinergic receptor, which activates a specific G protein, ____. This causes the dissociation of the trimeric G protein complex and the _____subunits bind to and activate a class of channels called _______, which OPEN/CLOSE (choose one) and result in ______ions moving INTO/OUT (choose one) of the cell. This DEPOLARIZES/HYPERPOLARIZES (choose one) the muscle cells, slowing the heartbeat.

Accepted Answer

The answer of Otto Loewi won a Noble Prize in...

Question 11

What are the two major/most common neurotransmitters in the vertebrate CNS?

Accepted Answer

A)  GABA and acetylcholine 
B)  Glycine and acetylcholine 
C)  GABA and glutamate 
D)  GABA and glycine

Question 12

In vertebrate CNS neurons, synapses are on dendritic spines. Most of the postsynaptic receptors are on the spine head, which are attached to the dendrite through a very small spine neck. What is the consequence of this small neck? Select all that apply.

Accepted Answer

A)  The creation of independent chemical and electrical compartments. 
B)  It creates the ability to modulate synaptic inputs independently. 
C)  It traps ions in specific locations in the neuron. 
D)  It creates the possibility that synaptic connections will be lost over time as they will break off.

Question 13

The efficacy of synaptic transmission can change. What best represents synaptic facilitation?

Accepted Answer

A)  A long-term increase in the number of synaptic contacts onto a postsynaptic neuron 
B)  An increase in the postsynaptic response to successive presynaptic action potentials 
C)  A decrease in the postsynaptic response to successive presynaptic action potentials 
D)  A decrease in the amplitude of the presynaptic action potential

Question 14

There are two main types of integration of signals, what are these and what is the difference between the two?

Accepted Answer

The answer of There are two main types of integration...

Question 15

When a trimeric G protein is activated, it dissociates into two protein complexes. What are these?

Accepted Answer

A)  Gα and Gβγ 
B)  Gαβ and Gγ 
C)  Gαγ and Gβ 
D)  Gβ and Gαδ

Question 16

Match between columns Premises: Muscarinic Kainate GABA_A GABA_B mGluR AMPA NMDA

Accepted Answer

The Answer of ionotropic glutamate receptor and metabotropic ACh receptor. is...

Question 17

When a depolarization occurs in the presynaptic terminal and Ca2+ channels open there is a large global rise in Ca2+ concentration. Defend your answer.

Accepted Answer

A) True 
 B)False

Question 18

Fill in the missing words in the following sentence.
PLC is activated by ______, a Gα variant. Activated PLC cleaves ______ to (1) ______, which in turn activates a serine/threonine kinase called ______, and (2) ______, which in turn binds to its receptor on the membrane of the endoplasmic reticulum (ER) and triggers the release of ______, interacting with an effector protein ______.

Accepted Answer

The answer of Fill in the missing words in the...

Question 19

Put the following in the correct order. A. Neurotransmitter release B. Ca²⁺ entry into the presynaptic terminal C. Axonal action potential D. Fusion of synaptic vesicle with the presynaptic plasma membrane E. Opening of voltage-gated Ca²⁺ channels F. Depolarization of the presynaptic terminal

Accepted Answer

The answer of Put the following in the correct order.
A....

Question 20

If you stimulate a glutamatergic presynaptic neuron and record the response in the postsynaptic neuron with NMDA receptors, what response will you get? Why?
(a) Depolarizing
(b) Hyperpolarizing
(c) No response

Accepted Answer

The answer of If you stimulate a glutamatergic presynaptic neuron...

Exam 3: Signaling Across Synapses

You take a 'normal' oocyte and apply ACh by iontophoresis to the oocyte and record the resulting current response with voltage clamp A. What will the resultant current be? Explain. B. You then inject the oocyte with mRNAs for AChR subunits and apply ACh. What will happen? Explain your answer.

Match each of the following statements that describe either:

Fill in the missing words in the following sentence. Glutamate is an amino acid. The neurotransmitter, _, is derived from glutamate by the enzyme _.

In adult neurons, there is a higher concentration of chloride outside the cell than inside the cell. During development intracellular chloride concentrations are much higher such that GABA causes excitation of the postsynaptic neuron. Why could changing the Cl^- concentration result in excitation?

How many ACh binding sites are there on each AChR?

Neurotransmitters can result in long-term changes in the physiological state of a neuron. One way this can happen is through activation of transcription factors, like CREB. How can neurotransmitters effect gene expression in neurons? Be as specific as possible.

What are the two major/most common neurotransmitters in the vertebrate CNS?

In vertebrate CNS neurons, synapses are on dendritic spines. Most of the postsynaptic receptors are on the spine head, which are attached to the dendrite through a very small spine neck. What is the consequence of this small neck? Select all that apply.

The efficacy of synaptic transmission can change. What best represents synaptic facilitation?

There are two main types of integration of signals, what are these and what is the difference between the two?

When a trimeric G protein is activated, it dissociates into two protein complexes. What are these?

Match

When a depolarization occurs in the presynaptic terminal and Ca²⁺ channels open there is a large global rise in Ca²⁺ concentration. Defend your answer.

Put the following in the correct order. A. Neurotransmitter release B. Ca²⁺ entry into the presynaptic terminal C. Axonal action potential D. Fusion of synaptic vesicle with the presynaptic plasma membrane E. Opening of voltage-gated Ca²⁺ channels F. Depolarization of the presynaptic terminal

If you stimulate a glutamatergic presynaptic neuron and record the response in the postsynaptic neuron with NMDA receptors, what response will you get? Why? (a) Depolarizing (b) Hyperpolarizing (c) No response

Exam 3: Signaling Across Synapses

You take a 'normal' oocyte and apply ACh by iontophoresis to the oocyte and record the resulting current response with voltage clamp A. What will the resultant current be? Explain. B. You then inject the oocyte with mRNAs for AChR subunits and apply ACh. What will happen? Explain your answer.

Match each of the following statements that describe either:

Fill in the missing words in the following sentence. Glutamate is an amino acid. The neurotransmitter, _______, is derived from glutamate by the enzyme _______.

In adult neurons, there is a higher concentration of chloride outside the cell than inside the cell. During development intracellular chloride concentrations are much higher such that GABA causes excitation of the postsynaptic neuron. Why could changing the Cl- concentration result in excitation?

How many ACh binding sites are there on each AChR?

Neurotransmitters can result in long-term changes in the physiological state of a neuron. One way this can happen is through activation of transcription factors, like CREB. How can neurotransmitters effect gene expression in neurons? Be as specific as possible.

What are the two major/most common neurotransmitters in the vertebrate CNS?

In vertebrate CNS neurons, synapses are on dendritic spines. Most of the postsynaptic receptors are on the spine head, which are attached to the dendrite through a very small spine neck. What is the consequence of this small neck? Select all that apply.

The efficacy of synaptic transmission can change. What best represents synaptic facilitation?

There are two main types of integration of signals, what are these and what is the difference between the two?

When a trimeric G protein is activated, it dissociates into two protein complexes. What are these?

Match

When a depolarization occurs in the presynaptic terminal and Ca2+ channels open there is a large global rise in Ca2+ concentration. Defend your answer.

Put the following in the correct order. A. Neurotransmitter release B. Ca2+ entry into the presynaptic terminal C. Axonal action potential D. Fusion of synaptic vesicle with the presynaptic plasma membrane E. Opening of voltage-gated Ca2+ channels F. Depolarization of the presynaptic terminal

If you stimulate a glutamatergic presynaptic neuron and record the response in the postsynaptic neuron with NMDA receptors, what response will you get? Why? (a) Depolarizing (b) Hyperpolarizing (c) No response

Fill in the missing words in the following sentence. Glutamate is an amino acid. The neurotransmitter, _, is derived from glutamate by the enzyme _.

In adult neurons, there is a higher concentration of chloride outside the cell than inside the cell. During development intracellular chloride concentrations are much higher such that GABA causes excitation of the postsynaptic neuron. Why could changing the Cl^- concentration result in excitation?

When a depolarization occurs in the presynaptic terminal and Ca²⁺ channels open there is a large global rise in Ca²⁺ concentration. Defend your answer.

Put the following in the correct order. A. Neurotransmitter release B. Ca²⁺ entry into the presynaptic terminal C. Axonal action potential D. Fusion of synaptic vesicle with the presynaptic plasma membrane E. Opening of voltage-gated Ca²⁺ channels F. Depolarization of the presynaptic terminal